Spectrophotometric Estimation of Levofloxacin Via Ion-Pair Complex Formation with Erythrosine B.

In this study, a simple, rapid, and cost-effective analytical method was described for the quantitative determination of levofloxacin (LEV) in its pure state and in therapeutic doses. The method relies on forming an ion-pair complex between LEV and erythrosine dye (ERY) at pH=4.0. The spectrum of the coloured product exhibits maximum absorption at 558 nm against the blank. A linear relationship, was obtained in the concentration range of 1.0-15.0 g/ml with a quantification coefficient of 0.9991 under the optimum conditions. The molar absorption coefficient and Sandells sensitivity index values were 1.91104 L/mol.cm and 0.0189 g/cm2 respectively, whereas the values of the detection limit and the quantification limit were 0.200 and 0.666 g/ml respectively. The developed method was effectively applied to estimate LEV present in pharmaceutical dosage. The accuracy and reliability of the suggested method were ascertained by recovery study via the standard addition method.