Molecular Characterization of Bacteria Isolated from Root Nodules of Lupinus Albus and Determination of its Family Specialization.

An endophytic bacteria isolated from root nodules of white
lupin (Lupinus albus) on agar solidified yeast extract mannitol
(YEM), after 48 hours, were white in color, with sticky appearance,
and showed the ability of mucus production. Isolated bacteria were
positive to oxidase and catalase and negative to gram stain. The
bacteria were resistance to 30µg/ml metronidazole antibiotic.
According to the diagnosis of the isolate at the molecular level by
using DNA sequencing technique for the analysis of 16S rRNA
gene with the global database, national center for biotechnology
information (NCBI), the results revealed 99.26% similarity of the
isolate in this study to the genus Rhizobium sp. were found in gene
bank, and its recorded for the first times as Rhizopium sp. ZAZ
strain in NCBI and the results showed responded of the white lupin
seedling grown on nitrogen free medium (NF) to inoculated with
Rhizobium sp. ZAZ isolate at 30 min and successful to form nodules
on roots in the rate of 65% and the rate number of nodes/seedling
was 2.3.